[略歴]
2005年　九州工業大学大学院博士後期課程修了（情報工学博士）
2005年　岡崎統合バイオサイエンスセンター　研究員
2008年　大阪大学大学院医学研究科統合生理学　研究員
2011年　京都大学大学院工学研究科分子生物化学分野　助教
2017年　現職 　　　　　

[研究テーマ] 　　　　　　
イオンチャネルの構造と生理機能

[趣味]
模索中 　　　


＜これまでの業績＞

[原著論文]

19)      Numata T, Tsumoto K, Yamada K, Kurokawa T, Hirose S, Nomura H, Kawano M, Kurachi Y, Inoue R, Mori Y. Integrative approach with electrophysiological and theoretical methods reveals a new role of S4 positively charged residues in PKD2L1 channel voltage-sensing. Sci. Rep. 7, 9760 (2017)

18)      Hirano M, Takada Y, Wong CF, Yamaguchi K, Kotani H, Kurokawa T, Mori MX, Snutch TP, Ronjat M, Waard M, Mori Y. C-terminal splice variants of P/Q-type Ca²⁺ channel CaV2.1 α1 subunits are differentially regulated by Rab3-interacting molecule proteins. J. Biol. Chem. 292, 9365-9381 (2017)

17)      Ogawa N, Kuroakwa T, Fujiwara K, Polat OK, Badr H, Takahashi N, Mori Y. Functional and structural divergence in Human TRPV1 channel subunits by oxydative cysteine modification. J. Biol. Chem. 291, 4197-4210 (2016)

16)      Sakata S, Miyawaki N, McCormack TJ, Arima H, Kawanabe A, Ozkucur N, Kurokawa T, Jinno Y, Fujiwara Y, Okamura, Y. Comparison between mouse and sea urchin orthologs of voltage-gated proton channel suggests role of S3 segment in activation gating. Biochim. Biophys. Acta 1858, 2972-2983 (2016)

15)      Takaya J, Mio K, Shiraishi T, Kurokawa T, Otsuka S, Mori Y, Uesugi M. A potent and site-selective of TRPA1. J. Am. Chem. Soc. 137, 15859-15864 (2015)

14)      Kurokawa T, Okamura Y. Mapping of sites facing aqueous environment of voltage-gated proton channel at resting state: A study with PEGylation protection. Biochim. Biophys. Acta 1838, 382-387 (2014)

13)      Takeshita K, Sakata S, Yamashita E, Fujiwara Y, Kawanabe A, Kurokawa T, Okochi Y, Matsuda M, Narita H, Okamura Y, Nakagawa A. X-ray crystal structure of voltage-gated proton channel. Nature Struct. Mol. Biol. 21, 352-357 (2014)

12)      Fujiwara Y, Kurokawa T, Okamura Y. Long alpha helices projecting from the membrane as the dimer interface in the voltage-gated H⁺ channel. J. Gen. Physiol. 143, 377-386 (2014)

11)      Yamaguchi S, Kurokawa T, Taira I, Aoki N, Sakata S, Okamura Y, Homma KJ. Potential role of voltage-sensing phosphatases in regulation of cell structure through the production of PI(3,4)P₂. J. Cell. Physiol. 229, 422-433 (2014)

10)    Fujiwara Y, Kurokawa T, Takeshita K, Nakagawa A, Larsson HP, Okamura Y. Gating of the Designed Trimeric/Tetrameric Voltage-Gated H⁺ Channel. J. Physiol. 591, 627-640 (2013)

9)    Kurokawa T, Takasuga S, Sakata S, Yamaguchi S, Horie S, Homma KJ, Sasaki T, Okamuka Y. 3′ Phosphatase activity toward phosphatidylinositol 3,4-bisphosphate [PI(3,4)P₂] by voltage-sensing phosphatase (VSP). Proc. Natl. Acad. Sci. USA 109, 10089-10094 (2012)

8)    Fujiwara Y, Kurokawa T, Takeshita K, Kobayashi M, Okochi Y, Nakagawa A, Okamura Y. The cytoplasmic Coiled-Coil Mediates cooperative gating temperature sensitivity in the voltage-gated H⁺ channel Hv1. Nature Commun. 3, 816 (2012)

7)    Matsuda M, Takeshita K, Kurokawa T, Sakata S, Suzuki M, Yamashita E, Okamura Y, Nakagawa A. Crystal structure of the cytoplasmic PTEN-like region of Ci-VSP provides insight into substrate specificity and redox regulation of the phosphoinositide phosphatase activity. J. Biol. Chem. 286, 23368-23377 (2011)

6)    Sakata S*, Kurokawa T*, Nørholm MH*, Takagi M, Okochi Y, von Heijne G, Okamura Y. Functionality of the voltage-gated proton channel truncated in S4. Proc. Natl. Acad. Sci. USA 107, 2313-2318 (2010) *)Contributed equally to this work

5)    Kishikawa J, Kabashima Y, Kurokawa T, Sakamoto J. The cytochrome bcc-aa₃-type respiratory chain of Rodococcus rhodochrous. J. Biosci. Bioeng. 110, 42-47 (2010)

4)    Kabashima Y, Kishikawa J, Kurokawa T, Sakamoto J. Correlation between proton translacation and growth: Genetic analysis of the respiratoty chain of Corynebacterium glutamicum. J. Biochem. 146, 845-855 (2009)

3)    Koch HP*, Kurokawa T*, Okochi Y, Sasaki M, Okamura Y, Larsson HP. Multimeric nature of voltage-gated proton channels. Proc. Natl. Acad. Sci. USA 105, 9111-9116 (2008) *)Contributed equally to this work

2)    Kurokawa T, Sakamoto J. Purification and characterization of succinate:menaquinone oxidoreductase from Corynebacterium glutamicum. Arch. Microbiol. 183, 317-324 (2005)

1)    Uchida T, Tsubaki M, Kurokawa T, Hori H, Sakamoto J, Kitagawa T, Sone N. Active site structure of SoxB-type cytochrome bo₃ oxidase from thermophilic Bacillus. J. Inorg. Biochem. 82, 65-72 (2000)

 [総説]

7)      Mori Y, Takahashi N, Kurokawa T, Kiyonaka S. TRP channels in oxygen physiology: distinctive functional properties and roles of TRPA1 in O₂ sensing. Proc. Jpn. Acad. Ser. B Phys. Biol. Sci. 93, 464-482 (2017)

6)      Ogawa N, Kuroakwa T, Mori Y. Sensing of redox status by TRP channels. Cell Calcium 60, 115–122 (2016)

5)      Mori Y, Takahashi N, Polat OK, Kuroakwa T, Takeda N, Inoue M. Redox-sensitive transient receptor potential channels in oxygen sensing and adaptation. Pflugers Arch. 468, 85–97 (2016)

4)      黒川竜紀, 森泰生 レドックスストレスを感知するイオンチャネルTRP アンチ・エイジング医学 11, 73–80 (2015)

3)      黒川竜紀, 森泰生 カチオン・カルシウムチャネルの農薬ターゲットとしての実際と可能性 日本農薬学会誌 40, 68–74 (2015)

2)      Mori MX, Itsuki K, Hase H, Sawamura S, Kurokawa T, Mori Y, Inoue R. Dynamics of receptor-operated Ca²⁺ currents through TRPC channels controlled via the PI(4,5)P₂-PLC signaling pathway. Front. Pharmacol. 6, 22 (2015)

1)      黒川竜紀, 岡村康司 電位依存性プロトンチャネルに見る膜電位センサーの謎：細胞膜中に存在する電荷 生物物理 51, 072-075 (2011)