講師(学内) ： 佐々木隆子 研究テーマ ： 細胞外マトリックスたんぱく質の機能とその構造との相関 研究内容 ： こ れまで、細胞外マトリックスたんぱく質の中で、特に基底膜を構成するたんぱく質（ラミニン、ナイドジェン、パーレカン、BM-40/SPARC, XV型ならびにXVIII型コラーゲン）の解析を組み換えたんぱく質を用い、たんぱく質相互作用ならびに作用部位の同定等の解析を行ってきた。最近は、比 較的新しい細胞外マトリックスたんぱく質ファミリーであるfibulin familyに属するたんぱく質の機能解析をin vitroの系のみならず、マウスモデルを用いても研究を遂行中である。 1) Surmann-Schmitt C, Sasaki T, Hattori T, Eitzinger N, Schett G, von der Mark K and Stock M (2012) The Wnt antagonist Wif-1 interacts with CTGF and inhibits CTGF activity. J. Cell Physiol., 227, 2207-2216. 2) Moriya K, Bae E, Honda K, Sakai K, Sakaguchi T, Tsujimoto I, Kamisoyama H, Keene DR, Sasaki T and Sakai T (2011) A fibronectin-independent mechanism of collagenfibrillogenesis in adult liver remodeling. Gastroenterology, 140, 1653-1663. 3) Sasaki T, Takagi J, Giudici C, Yamada Y, Arikawa-Hirasawa E, Deutzmann R, Timpl R, Sonnenberg A, Bächinger HP, Tonge D (2010) Laminin-121 – recombinant expression and interactions with integrins. Matrix Biol., 29, 484-493. 4) McCulloch DR, Nelson CM, Dixon LJ, Wylie JD, Lindner V, Sasaki T, Cooley MA,  Argraves WS, Apte SS (2009) ADAMTS metalloproteases generate active versican fragments that regulate interdigital web regression. Dev. Cell, 17, 687-698. 5) Hohenester E, Sasaki T, Giudici C, Farndale RW and Bächinger HP (2008)  Structural basis of sequence-specific collagen recognition by SPARC.Proc. Natl. Acad. Sci, USA, 105, 18273-18277. 6) Giudici C, Raynal N, Wiedemann H, Cabral WA, Marini JC, Timpl R, Bächinger HP, Farndale RW, Sasaki T and Tenni R (2008) Mapping of SPARC/BM-40/osteonectin-binding sites on fibrillar collagens. J. Biol. Chem., 283, 19551-19560. 7) Kobayashi N, Kostka G, Garbe, JHO, Keene DR, Bächinger HP., Hanisch F-G, Markova D, Tsuda T, Timpl R, Chu M-L and Sasaki T (2007)  A comparative analysis of the fibulin protein family: Biochemical characterization, binding interactions and tissue localization. J. Biol. Chem., 282, 11805-11816. 8) Sasaki T, Knyazev PG, Clout NJ, Cheburkin Y, Göhring W, Ullrich A, Timpl R and Hohenester E (2006) Structural basis for Gas6-Axl signalling.EMBO J, 25, 80-87. 9) Sasaki T, Fässler R, and Hohenester E (2004). Laminin: the crux of basement membrane assembly. J. Cell Biol., 164:959-963. Minireview 10) Timpl R, Sasaki T, Kostka G and Chu M-L (2003). Fibulins: a versatile family of extracellular matrix proteins. Nat Rev Mol Cell Biol. 4, 479-489. Review.