大分大学医学部感染予防医学講座

　Benjawan Saechue (Fai)

	Benjawan Saechue (D.V.M) 大分大学医学部感染予防医学講座　大学院生	Chikungunya fever is a mosquito-borne disease cause of persistent arthralgia. The current diagnosis of Chikungunya virus (CHIKV) relies on a conventional reverse transcription polymerase chain reaction assay. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a rapid and simple tool used for DNA-based diagnosis of a variety of infectious diseases. We established an RT-LAMP system to recognize CHIKV by targeting the envelope protein 1 (E1) gene which could also detect CHIKV at a concentration of 8 PFU without incorrectly detecting other mosquito-borne viruses. The system also amplified the E1 genome in the serum of CHIKV-infected mice with high sensitivity and specificity. Moreover, we established a dry RT-LAMP system that can be transported without a cold chain, which detected the virus genome in CHIKV-infected patient samples with high accuracy. Therefore, the dry RT-LAMP system has great potential to be applied as a novel CHIKV screening kit in endemic areas.

Benjawan Saechue (D.V.M)

大分大学医学部感染予防医学講座　大学院生

　Chikungunya fever is a mosquito-borne disease cause of persistent arthralgia. The current diagnosis of Chikungunya virus (CHIKV) relies on a conventional reverse transcription polymerase chain reaction assay. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) is a rapid and simple tool used for DNA-based diagnosis of a variety of infectious diseases. We established an RT-LAMP system to recognize CHIKV by targeting the envelope protein 1 (E1) gene which could also detect CHIKV at a concentration of 8 PFU without incorrectly detecting other mosquito-borne viruses. The system also amplified the E1 genome in the serum of CHIKV-infected mice with high sensitivity and specificity. Moreover, we established a dry RT-LAMP system that can be transported without a cold chain, which detected the virus genome in CHIKV-infected patient samples with high accuracy. Therefore, the dry RT-LAMP system has great potential to be applied as a novel CHIKV screening kit in endemic areas.

Nationality:
　　　Thailand 　　

Research Project
- Development of a portable reverse transcription Loop-mediated isothermal amplification system to detect the E1 region of Chikungunya virus in a cost-effective manner
- Simple diagnosis for Leishmania infection using a Cold chain-free loop-mediated isothermal amplification (LAMP)
- CRISPR/Cas9 Genome Editing in Mouse using i-GONAD method (improved-Genome editing via Oviductal Nucleic Acids Delivery)

Supervisor
　 Dr. Kamiyama, Prof. Kobayashi

Eudcation and Professional Experience
　October 2017 - September 2021: Oita University Graduate School of Medicine
　June 2011 - April 2017: Faculty of Veterinary Science, Mahasarakham University (Thailand)

Scientific Society
　　The Molecular Biology Society of Japan 日本分子生物学会

Comments
I’m a PhD student here since 2017 by MEXT scholarship. I’m very appreciate to be here. I would like to thank Kobayashi sensei, who gave me a good opportunity to study in Japan. In my opinion, it is very convenient and comfortable to live and study in Oita. This city is really quiet, clean and calm, which provides a better environment for study. Everybody are so nice, kind and always willing to help for everything. So far, I have not only enhanced my knowledge in my field of research but I also have learned many helpful things through my daily life in Japan. This is a very meaningful and wonderful time in my life. Moreover, it is my desire to see more Japan and enjoy my photography as well^^! 　

Place : Oita Prefecture
Photo by Faii_BS