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氏名:Benjawan Saechue
所属:感染予防医学講座
修学指導教員氏名:花田 俊勝、研究指導教員氏名:小林 隆志
演題目:Development of a rapid, simple and portable reverse transcription loop-mediated isothermal amplification method for Chikungunya virus.
座長:安東 優(呼吸器感染症内科学)・松本 昂(環境予防医学講座)
Chikungunya fever is a mosquito-borne disease that causes persistent arthralgia. Chikungunya virus (CHIKV) diagnostic assay currently relies on conventional reverse transcription polymerase chain reaction (RT-PCR) method that require the highly skilled personnel and precision instruments. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method is a rapid and simple tool used for the DNA-based diagnosis of a variety of infectious diseases. Therefore, we established a RT-LAMP system for CHIKV targeting the envelope protein 1 (E1) gene and the cold chain-free dried reagents of RT-LAMP system. |