◆2024年の研究室配属

【一言】
Nanさんとがんばります！

【一言】研究は大変なこともありますが、その分楽しいこともたくさんあります！充実した研究生活を送れるよう、鹿子嶋先生と一緒に頑張ります！

●　CRISPR-Cas9システムを用いたトキソプラズマの新規病原因子の探索
　　指導教員：神山、小林
　CRISPR-Cas9システムを用いて候補遺伝子を欠損したトキソプラズマ株を作製し、マウスへ感染させる。免疫学的手法による病態解析を実施し、トキソプラズマの病原性を規定する新規分子の探索を行う。
細胞内寄生原虫であるトキソプラズマ（T. gondii）は、妊婦が初感染した場合、胎児の水頭症や流産などの重篤な症状を引き起こす場合がある。これは先天性トキソプラズマ症と呼ばれ、我が国での原因不明の流産などの原因ではないかと考えられる。しかし、ワクチンを含む有効な予防法はまだ開発されていない。従って、T. gondiiの病原因子の理解とそれを標的とした新規予防法の開発が急務である。そこで、T. gondiiの新規病原因子を同定するために、高病原株と弱毒株の遺伝子発現量を比較し、高病原株で高発現している候補遺伝子のノックアウト株を樹立した。このノックアウト株をマウスに感染させ、その病原性を評価する。

技術：CRISPR-Cas9、PCR法、マウス感染実験、病理学的解析、免疫染色

【一言】精一杯頑張ります！

●　タイ・マヒドン大学留学　Exploring characteristic gene expression in immune cells of Triple Negative Breast Cancer patients through single-cell RNA sequence
　　指導教員：Dr. Varodom Charoensawan (Siriraj Hosp, Mahidol Univ)、小林
　Triple negative breast cancer (TNBC) is a type with no marker proteins known to be associated with breast cancer cell growth. The cause of TNBC is not yet completely understood and is complicated by cellular heterogeneity of the cancer tissues and immune cells associated with cancer microenvironment.
In this study, we aim to explore biomarkers that characterize immune abnormalities in patients with TNBC in different through single-cell RNA sequencing (scRNA-seq).
　

技術：single-cell RNA sequence

【一言】滝が好きです

●　タイ・チュラロンコン大学留学 Verification of the ability of Leishmania to induce trained immunity
　　指導教員：神山、小林, Prof. Tanapat Palaga
　The adoptive immunity is known to have an immune memory effect and can induce a stronger immune response to a second or subsequent infection than to a first one. In contrast, it is believed that the innate immunity does not have immune memory. However, it is becoming clear that the innate immune system also has immune memory, albeit for a short period. This is called "trained immunity" and is induced by epigenetic changes, and we have shown that O6-methylguanine DNA methyltransferase (MGMT), a DNA repair enzyme, is involved in the induction of trained immunity by β-glucan. In this study, we aimed to verify whether Leishmania, which causes cutaneous and visceral leishmaniasis through the vector of the sand fly, is capable of inducing trained immunity, and to clarify whether MGMT is involved in the induction of trained immunity.
　

技術：Cell culture, ELISA, real time PCR

【一言】

指導教員：花田礼子教授
【Reseach theme】When i internship in oita university were beautiful moment and precious time. One month of internship i assigned in department of neurophysiology under Prof. Hanada and Dr. Magdeline Carrasco for supervisor. I joined the journal club for first time. Which is i got useful commention and advices for improvement in next journal club or seminar. For the experiment while i taken in the internship, this associate about dangerous effect of alcohol on the liver while have fatty liver complication (alcohol- induced hepatic steatosis/ fatty liver disease). My experiment focus on Beta-hydroxybutyrate (as known as weight lose drug) have effect to reduce liver damage from alcohol metabolic product releasing, which is ATP/Adenosine in zebrafish liver. The result quite interest because Beta-hydroxybutyrate can reduce ATP/Adenosine releasing. That can develope of drug for treatment or prevent liver from hepatic steatosis but must concider about useful level. This drug can induce ketosis.

【Comment】
And i learn about zebrafish behavior, water management and seperate male and female fish. At first i taugh that difficult to seperated but Umeda-san, Magdeline-san teach me how to seperated. And finally, i can sperated them by myself!. Moreover, Many tecnique are new thing for me. They taugh me how to used mechine. After i can used mechine, can i determined the required information? It's yes or not? Which i can determined required information by they help me
In addition, i lectured a class with 2nd year medical student in Autonomic nervous system topic. Other experience about life in Oita, I have new friend from another Thai university and Japaness friend. The new friendship that precious one in my life. New friend make me came out of comfortable zone, try to communicated, try to done new thing. If i did not came here, i never known this one. There are precious memories. I want to say thank you for everyone which is help me in this internship. I don't know how to say expect THANK YOU for this experience.

指導教員：花田礼子教授
What a wonderful month I just had here at Oita University. I sure gained a lot of knowledge and experience from this visit, yet what I am most grateful for are all the incredible people I have met here. It was a moment I will always cherish. Special shoutout to the Neurophysiology Lab people—you guys are the best, and I could not ask for more !
 
They say that travel changes you. I am not sure how much of an impact I received here, but I sure like how it turned out.

ありがとう、大分 :>

Tutor-kun Preem-san and Rally-san, second year medical students at Siriraj Hospital, Mahidol University, Thailand, joined us on April 1st. They participate in our program of research training. Tutor-kun studys on autoimmune diseases using mouse models with Nan-san in our lab. Preem-san joined R.Hanada's Lab and Rally-san joined T. Hanada's lab.

On Sunday, April 7th, Yuri Shiina guided students from the Faculty of Medicine of Mahidol University to Yufuin.

We saw water fall, Ryushonotaki, in Shonai town. We then visited Yufuin town where we saw the Kinrinko lake and visited the Tenso shrine. We went shopping on the Yunotsubo-kaido street.